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Table 2 Plasmids

From: Dynamic regulation of gene expression using sucrose responsive promoters and RNA interference in Saccharomyces cerevisiae

Name Details Origin
pRS406 URA3 integrating vector [45], Euroscarf
pSF019 TEF1 promoter containing vector [29]
yEGFPCLN2PEST-pRS406 Destabilized GFP base plasmid [36]
PMAL12- yEGFPCLN2PEST-pRS406 MAL12 promoter driven GFP expression This study
PGLC3- yEGFPCLN2PEST-pRS406 GLC3 promoter driven GFP expression This study
PGPH1- yEGFPCLN2PEST-pRS406 GPH1 promoter driven GFP expression This study
PSUC2- yEGFPCLN2PEST-pRS406 SUC2 promoter driven GFP expression This study
PTEF1-yEGFPCLN2PEST-406 Constitutive TEF1 promoter driven GFP expression This study
pRS413 Yeast centromeric plasmid with HIS3 selection marker [45], Euroscarf
CYC1t-pRS413 CYC1 terminator This study
PSUC2-CYC1t-pRS413 SUC2 promoter, CYC1 terminator This study
PSUC2-GFPantisense-CYC1t-pRS413 SUC2 regulated expression of an antisense RNAi construct for GFP This study
pRS404-PTEF -Ago1-CYC1t TRP1 integrating vector with constitutive Argonaute expression [5]
pRS405-PTEF-Dcr1-CYC1t LEU2 integrating vector with constitutive Dicer expression [5]
pUG6 LoxP-KanMX-LoxP cassette Euroscarf
pUG66 LoxP-Ble-LoxP cassette Euroscarf