Table 2 Plasmids
Name | Details | Origin |
|---|---|---|
pRS406 | URA3 integrating vector | [45], Euroscarf |
pSF019 | TEF1 promoter containing vector | [29] |
yEGFPCLN2PEST-pRS406 | Destabilized GFP base plasmid | [36] |
PMAL12- yEGFPCLN2PEST-pRS406 | MAL12 promoter driven GFP expression | This study |
PGLC3- yEGFPCLN2PEST-pRS406 | GLC3 promoter driven GFP expression | This study |
PGPH1- yEGFPCLN2PEST-pRS406 | GPH1 promoter driven GFP expression | This study |
PSUC2- yEGFPCLN2PEST-pRS406 | SUC2 promoter driven GFP expression | This study |
PTEF1-yEGFPCLN2PEST-406 | Constitutive TEF1 promoter driven GFP expression | This study |
pRS413 | Yeast centromeric plasmid with HIS3 selection marker | [45], Euroscarf |
CYC1t-pRS413 | CYC1 terminator | This study |
PSUC2-CYC1t-pRS413 | SUC2 promoter, CYC1 terminator | This study |
PSUC2-GFPantisense-CYC1t-pRS413 | SUC2 regulated expression of an antisense RNAi construct for GFP | This study |
pRS404-PTEF -Ago1-CYC1t | TRP1 integrating vector with constitutive Argonaute expression | [5] |
pRS405-PTEF-Dcr1-CYC1t | LEU2 integrating vector with constitutive Dicer expression | [5] |
pUG6 | LoxP-KanMX-LoxP cassette | Euroscarf |
pUG66 | LoxP-Ble-LoxP cassette | Euroscarf |