Table 1 Mild solubilization methods used for recovery of proteins from inclusion bodies

Tris-Cl buffer, low concentration of DMSO, n-propanol and Sarcosyl

Non-denaturing solubilization methods for non-classical inclusion bodies. No need of refolding

[46,62]

Low concentration of urea

Mild solubilization, low concentration of urea doesn’t completely denature solubilized protein molecules

[46,63]

Organic solvent based solubilization

High solubilization efficiency, inhibition of aggregation during refolding

[60]

High hydrostatic pressure

High solubilization efficiency with simultaneous refolding of solubilized protein

[64,68]

High pH buffers

High pH in combination with low concentration of urea. Mild, efficient solubilization

[19,61]

Detergents

Mild solubilization

[65,66]