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Strains
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E. coli BL21(DE3)
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F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
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New England Biolabs
|
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E. coli BL21(DE3)-RIL plus
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F– ompT hsdS(rB– mB–) dcm + Tetr gal λ(DE3) endA Hte [argU ileY leuW Camr]
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Agilent Technologies
|
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S. cerevisiae BY4742
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MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0
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GE Healthcare
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Plasmids
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pGFP
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pET-28b(+) derivative with gfp insertion
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This study
|
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pATF1G
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pET-28b(+) derivative with atf1(S.c)-gfp insertion
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This study
|
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pATF2G
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pET-28b(+) derivative with atf2(S.c)-gfp insertion
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This study
|
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pSPG
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pET-28b(+) derivative with atf(S.p)-gfp insertion
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This study
|
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pKLG
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pET-28b(+) derivative with atf(K.l)-gfp insertion
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This study
|
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pPAG
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pET-28b(+) derivative with atf(P.a)-gfp insertion
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This study
|
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pSLG
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pET-28b(+) derivative with atf(S.l)-gfp insertion
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This study
|
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pYPGK
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pRS426 derivative; PGK1p-PGK1t
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[19]
|
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pYATF1G
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pYPGK derivative with atf1(S.c)-gfp insertion
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[19]
|
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pYATF2G
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pYPGK derivative with atf2(S.c)-gfp insertion
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[19]
|
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pYSPG
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pYPGK derivative with atf(S.p)-gfp insertion
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[19]
|
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pYKLG
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pYPGK derivative with atf(K.l)-gfp insertion
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[19]
|
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pYPAG
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pYPGK derivative with atf(P.a)-gfp insertion
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[19]
|
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pYSLG
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pYPGK derivative with atf(S.l)-gfp insertion
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[19]
|
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Primers
| | |
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gfp
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5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGCTAGCATGACTGGTG3'
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This study
|
| |
5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3'
|
This study
|
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atf1-S.c-gfp
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5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGAATGAAATCGATGAGAAAAATCAG3'
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This study
|
| |
5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3'
|
This study
|
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atf2-S.c-gfp
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5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGAAGATATAGAAGGATACGAACCACATATCACTC3'
|
This study
|
| |
5'ACGCGTCGACTTATTTGTATAGTTCATCCATGCCATG3'
|
This study
|
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atf-S.p-gfp
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5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGAAACAGAAGAAAGCCAATTTAGCAGTATAAC3'
|
This study
|
| |
5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3'
|
This study
|
|
atf-K.l-gfp
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5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGGTTCGGTGTGTTTATCATCAAAAAAGTTAG3'
|
This study
|
| |
5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3'
|
This study
|
|
atf-P.a-gfp
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5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGTTGTTAAATTCAAAAGCAAAATCAATAACAAAGG3'
|
This study
|
| |
5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3'
|
This study
|
|
atf-S.l-gfp
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5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGCTAATATTTTGCCAATTTC3'
|
This study
|
| |
5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3'
|
This study
|
