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Table 1 Strains, plasmids and primers used in this study

From: Microbial host selection affects intracellular localization and activity of alcohol-O-acetyltransferase

Name

Description

Source

Strains

  

E. coli BL21(DE3)

F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

New England Biolabs

E. coli BL21(DE3)-RIL plus

F– ompT hsdS(rB– mB–) dcm + Tetr gal λ(DE3) endA Hte [argU ileY leuW Camr]

Agilent Technologies

S. cerevisiae BY4742

MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0

GE Healthcare

Plasmids

  

pGFP

pET-28b(+) derivative with gfp insertion

This study

pATF1G

pET-28b(+) derivative with atf1(S.c)-gfp insertion

This study

pATF2G

pET-28b(+) derivative with atf2(S.c)-gfp insertion

This study

pSPG

pET-28b(+) derivative with atf(S.p)-gfp insertion

This study

pKLG

pET-28b(+) derivative with atf(K.l)-gfp insertion

This study

pPAG

pET-28b(+) derivative with atf(P.a)-gfp insertion

This study

pSLG

pET-28b(+) derivative with atf(S.l)-gfp insertion

This study

pYPGK

pRS426 derivative; PGK1p-PGK1t

[19]

pYATF1G

pYPGK derivative with atf1(S.c)-gfp insertion

[19]

pYATF2G

pYPGK derivative with atf2(S.c)-gfp insertion

[19]

pYSPG

pYPGK derivative with atf(S.p)-gfp insertion

[19]

pYKLG

pYPGK derivative with atf(K.l)-gfp insertion

[19]

pYPAG

pYPGK derivative with atf(P.a)-gfp insertion

[19]

pYSLG

pYPGK derivative with atf(S.l)-gfp insertion

[19]

Primers

  

gfp

5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGCTAGCATGACTGGTG3'

This study

 

5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3'

This study

atf1-S.c-gfp

5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGAATGAAATCGATGAGAAAAATCAG3'

This study

 

5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3'

This study

atf2-S.c-gfp

5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGAAGATATAGAAGGATACGAACCACATATCACTC3'

This study

 

5'ACGCGTCGACTTATTTGTATAGTTCATCCATGCCATG3'

This study

atf-S.p-gfp

5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGAAACAGAAGAAAGCCAATTTAGCAGTATAAC3'

This study

 

5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3'

This study

atf-K.l-gfp

5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGGTTCGGTGTGTTTATCATCAAAAAAGTTAG3'

This study

 

5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3'

This study

atf-P.a-gfp

5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGTTGTTAAATTCAAAAGCAAAATCAATAACAAAGG3'

This study

 

5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3'

This study

atf-S.l-gfp

5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGCTAATATTTTGCCAATTTC3'

This study

 

5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3'

This study