Name | Description | Source |
---|---|---|
Strains | Â | Â |
E. coli BL21(DE3) | F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) | New England Biolabs |
E. coli BL21(DE3)-RIL plus | F– ompT hsdS(rB– mB–) dcm + Tetr gal λ(DE3) endA Hte [argU ileY leuW Camr] | Agilent Technologies |
S. cerevisiae BY4742 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | GE Healthcare |
Plasmids | Â | Â |
pGFP | pET-28b(+) derivative with gfp insertion | This study |
pATF1G | pET-28b(+) derivative with atf1(S.c)-gfp insertion | This study |
pATF2G | pET-28b(+) derivative with atf2(S.c)-gfp insertion | This study |
pSPG | pET-28b(+) derivative with atf(S.p)-gfp insertion | This study |
pKLG | pET-28b(+) derivative with atf(K.l)-gfp insertion | This study |
pPAG | pET-28b(+) derivative with atf(P.a)-gfp insertion | This study |
pSLG | pET-28b(+) derivative with atf(S.l)-gfp insertion | This study |
pYPGK | pRS426 derivative; PGK1p-PGK1t | [19] |
pYATF1G | pYPGK derivative with atf1(S.c)-gfp insertion | [19] |
pYATF2G | pYPGK derivative with atf2(S.c)-gfp insertion | [19] |
pYSPG | pYPGK derivative with atf(S.p)-gfp insertion | [19] |
pYKLG | pYPGK derivative with atf(K.l)-gfp insertion | [19] |
pYPAG | pYPGK derivative with atf(P.a)-gfp insertion | [19] |
pYSLG | pYPGK derivative with atf(S.l)-gfp insertion | [19] |
Primers | Â | Â |
gfp | 5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGCTAGCATGACTGGTG3' | This study |
 | 5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3' | This study |
atf1-S.c-gfp | 5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGAATGAAATCGATGAGAAAAATCAG3' | This study |
 | 5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3' | This study |
atf2-S.c-gfp | 5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGAAGATATAGAAGGATACGAACCACATATCACTC3' | This study |
 | 5'ACGCGTCGACTTATTTGTATAGTTCATCCATGCCATG3' | This study |
atf-S.p-gfp | 5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGAAACAGAAGAAAGCCAATTTAGCAGTATAAC3' | This study |
 | 5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3' | This study |
atf-K.l-gfp | 5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGGTTCGGTGTGTTTATCATCAAAAAAGTTAG3' | This study |
 | 5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3' | This study |
atf-P.a-gfp | 5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGTTGTTAAATTCAAAAGCAAAATCAATAACAAAGG3' | This study |
 | 5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3' | This study |
atf-S.l-gfp | 5'GCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGCTAATATTTTGCCAATTTC3' | This study |
 | 5'CCGCTCGAGTTATTTGTATAGTTCATCCATGCCATG3' | This study |