Figure 5

Reduced AATase expression decreases aggregates size in E. coli fermentations. A) Fluorescence microscopy images and aggregate size measurement of Atf1-S.c and Atf-S.l in E. coli. Scale bar is 1 μm. Error bars represent standard deviation (n = 100), * p < 0.05. B, C) Relative expression levels of Atf1-S.c (B) and Atf-S.l (C) in E. coli as judged by western blot analysis. Expression levels are normalized to the intensity of anti-GFP signal from E. coli induced with 1 μM IPTG. D, E) Normalized AATase activity from Atf1-S.c (D) and Atf-S.l (E) during 24 hour E. coli fermentations at 30°C. Activities are normalized to the lowest expression level, IPTG induction of 1 μM. Error bars represent standard deviation (n = 3).