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Figure 3 | Microbial Cell Factories

Figure 3

From: Microbial host selection affects intracellular localization and activity of alcohol-O-acetyltransferase

Figure 3

Expression, intracellular localization, and activity of AATases. A) Fluorescence microscopy images of S. cerevisiae co-expressing AATase-GFP and the LD marker Erg6-DsRed. Expressed AATases include Atf2 from S. cerevisiae (Atf2-S.c), and AATases from S. pastorianus (Atf-S.p), K. lactis (Atf-K.l), P. anomala (Atf-P.a), and S. lycopersicum (Atf-S.l). Fluorescence from Erg6-DsRed is shown in red and fluorescence from AATase-GFP is shown in green. Overlapping Erg6-DsRed and GFP signals, indicating co-localization, are shown in yellow. Scale bar (1 μm) applies to A) and B). B) Fluorescent microscopy images of E. coli expressing AATases. CellMask™ Orange plasma membrane staining is shown in red and fluorescence from AATase-GFP is shown in green. Graphs below the fluorescence images indicate the number of aggregates observed in E. coli cells. A minimum of 100 cells were counted from three independent experiments. C) In vitro ethyl acetate production from whole cell lysates of AATase-GFP expressed in S. cerevisiae (S.c) and E. coli. Error bars represent standard deviation (n = 3). D) Western blot analysis of AATase-GFP expression in S. cerevisiae and E. coli.

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