Expression, intracellular localization, and activity of Atf1 from
(Atf1-S.c). A) Fluorescence microscopy images of S. cerevisiae co-expressing Atf1-GFP, GFP, and the LD marker Erg6-DsRed. Fluorescence from Erg6-DsRed is shown in red and fluorescence from Atf1-GFP is shown in green. Overlapping Erg6-DsRed and Atf1-GFP signals, indicating co-localization, are shown in yellow. GFP controls show cytosolic localization. Scale bar (1 μm) applies to A) and B). B) Fluorescent microscopy images of E. coli expressing Atf1-GFP and GFP. CellMask™ Orange plasma membrane staining is shown in red and fluorescence from Atf1-GFP and GFP is shown in green. Graphs below the fluorescence images indicate the number of aggregates observed in E. coli cells. A minimum of 100 cells were counted from three independent experiments. C) Western blot analysis of Atf1-GFP expression in S. cerevisiae (S.c) and E. coli. D)
In vitro ethyl acetate production from whole cell lysates of Atf1-GFP expressed in S. cerevisiae and E. coli. Error bars represent standard deviation (n = 3).