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Table 3 Oligonucleotides used for constructing plasmids and qPCR studies

From: Dynamic control of ERG9 expression for improved amorpha-4,11-diene production in Saccharomyces cerevisiae

Name

Discription

F_URA3_HindIII

AGAGAAAGCTTGCAGCGAGAACACGACCACGCCCAATACAACAGATCACGTG

R_URA3_BE

GGAATTCGGATCCAGGTTCTATCGAGGAGAAAAAGCG

F_ERG1p_BamHI

CGGGATCCGTCGAATACTACTATGACCG

R_ERG1p_EcoRI

GGAATTCCCAATTGTAATAGCTTTCCCATGACCCTTTTCTCGATATGTT

F_ERG11p_BamHI

CGGGATCCCTTGTTCTCTCTCGCTTCC

R_ERG11p_EcoRI

GGAATTCCCAATTGTAATAGCTTTCCCATCCTTGTATTACTCGTTTGTTC

F_ERG2p_BamHI

CGGGATCCAGTGTTAGCAAGCGCAGACG

R_ERG2p_EcoRI

GGAATTCCCAATTGTAATAGCTTTCCCATGGCTATAATGGTCTGGGCTAG

F_ERG3p_BamHI

CGGGATCCGAATATCGTCAACCTCGTCC

R_ERG3p_EcoRI

GGAATTCCCAATTGTAATAGCTTTCCCATATCTCAAATCTAGACGAATATT

F_ADS_BamHI

CGGGATCCAAAACAATGGCCCTGACCGAAGAG

R_ADS_XhoI

ACACGCTCGAGTCAGATGGACATCGGGTAAAC

F_ERG9p_Int

GGTTTTGGGTTTAGTGCCTAAACGAGCAGCGAGAACACGACCACG

R_ERG9p_Int

CTTCATCTCGACCGGATGCAATGCCAATTGTAATAGCTTTCCCAT

Primer for qPCR study

F_ACT1_q

TCCGTCTGGATTGGTGGT

R_ACT1_q

TGAGATCCACATTTGTTGGAAG

F_ERG1_q

TGTTGGTGCCAAGGTTGA

R_ERG1_q

AAATGTCAAGTGGGCTTTGAA

F_ERG11_q

TGCACGTTCCAAACACTTCT

R_ERG11_q

CCTGGAGAAACCAAAACGTG

F_ERG2_q

AATTGGCTCAAGGCTGGATT

R_ERG2_q

TGGAGAAAGTGTCCAAAAACC

F_ERG3_q

CAACTACGGTCAATTCACCACTC

R_ERG3_q

AATGAGTCATCTGGTCTACGGTAA

F_ERG9_q

TTACAATTGGCATTGCATCC

R_ERG9_q

TTCTGCAAAACTTCAGCTTCAA