Table 2 List of plasmids and strains used in the present study
Name | Description | References |
|---|---|---|
Plasmid name | ||
pUC18 | Plasmid for cloning in E. coli | Invitrogen |
pUG72 | Plasmid harboring URA3 selection marker | [30] |
pSH68 | Plasmid harboring Cre gene under the control of P GAL1 | [30] |
pRS425ADS | pRS425::P GAL1 -ADS-T CYC1 | [9] |
pRS415ADS | pSH68 derivative with pRS415::P GAL1 -ADS-T CYC1 | This study |
pURA3-Blank | pUC18 derivative containing URA3 selection marker from pUG72 | This study |
pURA3-ERG1p | pURA3-Blank derivative with insertion of promoter region from ERG1 gene | This study |
pURA3-ERG11p | pURA3-Blank derivative with insertion of promoter region from ERG11 gene | This study |
pURA3-ERG2p | pURA3-Blank derivative with insertion of promoter region from ERG2 gene | This study |
pURA3-ERG3p | pURA3-Blank derivative with insertion of promoter region from ERG3 gene | This study |
Strain name | ||
M4-2nd | BY4742 derivative with the relatively high mevalonate pathway activity | [3] |
M4-D1 | M4-2nd with ERG9 under the control of P ERG1 | This study |
M4-D2 | M4-2nd with ERG9 under the control of P ERG11 | This study |
M4-D3 | M4-2nd with ERG9 under the control of P ERG2 | This study |
M4-D4 | M4-2nd with ERG9 under the control of P ERG3 | This study |
M4-2nd-L | Strain M4-2nd harboring plasmid pRS415ADS | This study |
M4-D1-L | Strain M4-D1 harboring plasmid pRS415ADS | This study |
M4-D2-L | Strain M4-D2 harboring plasmid pRS415ADS | This study |
M4-D3-L | Strain M4-D3 harboring plasmid pRS415ADS | This study |
M4-D4-L | Strain M4-D4 harboring plasmid pRS415ADS | This study |