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Figure 4 | Microbial Cell Factories

Figure 4

From: Dynamic control of ERG9 expression for improved amorpha-4,11-diene production in Saccharomyces cerevisiae

Figure 4

qRT-PCR analysis of ERG9 expression in the engineered strains. Five cultures of each strain, M4-2nd, M4-D1, M4-D2, M4-D3 and M4-D4 were inoculated in SC media at initial OD600 of 0.05 and harvested during early-log phase for qRT-PCR analysis. All strains harboring plasmid pRS415ADS were subjected to qRT-PCR analysis to determine ERG9 expression levels in engineered strains. The results are presented as the relative abundances of ERG9 in each strain with respect to that of ACT1. Values represented the average and standard deviation of three independent experiments.

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