Figure 2
qRT-PCR analysis of ERG1 , ERG11 , ERG2 and ERG3 in an engineered strain with high mevalonate pathway activity over the reference strain BY4742. Two cultures of each strain, BY4742 (Control) and M4-2nd, were inoculated in SC media at initial OD600 of 0.05 and harvested at early-log phase for qRT-PCR analysis. The results are presented as the relative abundances of ERG1, ERG11, ERG2 and ERG3 in each strain with respect to ACT1. Values represented the average and standard deviation of three independent experiments.