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Figure 4 | Microbial Cell Factories

Figure 4

From: Bioprocess automation on a Mini Pilot Plant enables fast quantitative microbial phenotyping

Figure 4

Substrate uptake characteristics of C. glutamicum xylXABCD Cc during growth on CGXII medium with D-glucose and D-xylose. The cultivation was performed in 46 identically inoculated wells of a FlowerPlate, of which each well was harvested automatically following a time-dependent pattern (cf. Figure 1C). Supernatant samples were automatically clarified via centrifugation, stored at −4°C and subsequently analyzed for D-glucose and D-xylose concentrations (n = 3 technical replicates). The results of both enzymatic substrate quantification methods were confirmed with established HPLC protocols. Mean values for backscatter and dissolved oxygen were estimated from unsampled replicate cultures (n ≥ 3). Confidence intervals (shaded areas) were spanned from the minimum and maximum value at each measurement point.

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