Figure 2

Development and validation of metabolite quantification in microtiter plate scale on the MPP. A-C: Fast photometric quantification of amino acids was realized by the application of the developed Ninhydrin assay in 384-well microtiter plates. The assay was tested with regard to the measurable metabolite spectrum as well as its selectivity against relevant by-products and media components of the model host C. glutamicum. D-E: Enzymatic assays to quantify D-glucose or D-xylose in culture supernatants were automated in 384-well microtiter plates.