Table 2 Strains and plasmids used in this study
Strains and plasmids | Information | Source |
|---|---|---|
Strains | Â | Â |
Vibrio fischeri MJ11 | Wild type, used for cloning of luxR/I quorum sensing system | MCCC |
Edwardsiella tarda EIB202 | Wild type, fish pathogen, broad range testing host | Our lab |
Edwardsiella tarda WED | Mutant disrupted in type III secretion system and chorismic acid synthesis, a live attenuated vaccine | Our lab |
Escherichia coli BL21 | General expressing strain, broad range testing host | Novagen |
Vibrio anguillarum MVM425 | Wild type, fish pathogen, broad range testing host | Our lab |
Staphylococcus aureus | Wild type, human pathogen, broad range testing host | Our lab |
Salmonella typhimurium | Wild type, human pathogen, broad range testing host | Our lab |
Aeromonas hydrophila LSA34 | Gene source of protective antigen GAPDH | Our lab |
Plasmids | Â | Â |
pUTat | Expression vector, Ampr | Our lab [35] |
pQS | pUTat vector containing intact QS gene elements and a reporter gene co-transcribed with luxI | This work |
ironQS1 | pQS plasmid derivate in which the original P luxR promoter was substituted for a low-iron-triggered promoter P viuA | This work |
ironQS2 | pQS plasmid derivate inserted with a standard Fur box into −10 region of luxI promoter | This work |
ironQS3 | pQS plasmid derivate inserted with two standard Fur boxes into −10 region of luxI and luxR promoters | This work |
ironQS4 | pQS plasmid derivate inserted with two continuous Fur box into −10 region of luxI promoter | This work |
ironQS-G | ironQS plasmid in which reporter gene was substituted by gapA34 gene | This work |