Microbial Cell Factories

Table 1 Flux scenarios associated with macroscopically indistinguishable phenotypes*

From: Effect of plasmid replication deregulation via inc mutations on E. coli proteome & simple flux model analysis

Objective	Y _x/s	Y _x/ATP	r _TCA	r _PYK	r _HMP	r _ME	r _TA	r _TK1	r _TK2	r _ANA	r _CO2
1. MIN r _TCA	0.45	10	0.60	0.48	1.4	0	0.43	0.43	0.36	0.57	4.64
MIN r _GLU
MIN r _ME
MAX r _HMP
MAX r _TA
MAX r _TK2
MAX r ₄
MAX r ₅
2. MIN r _PYK	0.44	10	0.77	0	0.99	0.63	0.29	0.29	0.22	1.2	4.12
3. MAX r _ME	0.40	7.3	1.6	0	0.32	1.15	0.072	0.072	0	1.7	5.38
MAX r _ANA
MIN r _HMP
MIN r _TA
MIN r _TK2
MIN r ₄
MIN r ₅
4. MAX r _TCA	0.39	7.0	1.7	0.13	0.32	1.1	0.072	0.072	0	1.6	5.72
MAX r _GLU
5. MAX r _PYK	0.39	7.0	1.4	0.93	1.0	0	0.30	0.30	0.23	0.57	6.59
MIN r _ANA

*Symbols: cell yield on glucose (g cell/g glucose), Y_x/s; cell yield on ATP (g cell/mol ATP), Y_x/ATP. See Figure 1 for r _TCA , r _PYK , r _HMP , r _ME , r _TA , r _TK1 , r _TK2, and r _ANA; r _CO2 is the net carbon dioxide flux. Flux units are mmol/g cell h. Bolded scenarios 3 and 4 indicate potential transformant solutions.

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ISSN: 1475-2859

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