Figure 5
Summary overlay of the current and prior studies for plasmid-containing cells. The upregulated (apex-up, white triangles) and downregulated (apex-down, black triangles) metabolic enzymes found in this study as an adaptation to deregulated plasmid replication are shown. Also shown are annotations to enzymes that others have upregulated or knocked out in order to assess the effect on plasmid copy number or improve the tolerance to plasmid burden. Based on computed flux scenarios and projections from the proteomics, large arrows depict flux change possibilities for cells with deregulated plasmid replication as compared to untransformed cells; green, red, and white arrow colors correspond to increased, decreased, and not distinguishably different fluxes.