Figure 1

Metabolic network for flux analysis. Network used to model the central metabolism of E. coli. Metabolite abbreviations are αKG: α-ketoglutarate; 2PG: 2-phosphoglycerate; 3PG: 3-phosphoglycerate; 6PG: 6-phosphogluconate; 6PGL: 6-phosphogluconolactone; AcCoA: acetyl-coenzyme A; BPG: 1,3-bisphosphoglycerate; CIT: citrate; DHAP: dihydroxyacetone phosphate; E4P: erythrose 4-phosphate; F6P: fructose-6-phosphate; FBP: fructose-1,6-bisphosphate; FUM: fumarate; G6P: glucose-6-phosphate; GAP: glyceraldehyde phosphate; ICIT: isocitrate; MAL: malate; OAA: oxaloacetate; PEP: phosphoenolpyruvate; PYR: pyruvate; R5P: ribose-5-phosphate; Ru5P: ribulose-5-phosphate; S7P: sedoheptulose-7-phosphate; SUC: succinate; S-CoA: succinyl-coenzyme A; and X5P: xylulose-5-phosphate. Specific fluxes shown are those associated with glucose uptake (r _GLU ), hexose monophosphate pathway (r _HMP ), transaldolase (r _TA ), transketolase (r _TK1 , r _TK2 ), tricarboxylic acid cycle (r _TCA ), pyruvate kinase (r _PYK ), malic enzyme (r _ME ), anaplerotic replenishment of OAA (r _ANA ), and formation of lactate and acetate (r _LAC , r _ACE ).