Figure 5

HPLC chromatogram of the cyproterone acetate conversion using B. megaterium MS941 overexpressing the CYP106A2 enzyme. The reaction was performed with resting cells in 100 mM potassium phosphate buffer (pH 7.4) at 30°C, 150 rpm. Cyproterone acetate (400 μM) was added to the cells in DMSO solution (2% v/v). Samples were collected directly at the point of substrate addition (grey dotted line) and after 2 h (black line). The substrate was converted to one main product (P1).