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Figure 3 | Microbial Cell Factories

Figure 3

From: Process development for the production of 15β-hydroxycyproterone acetate using Bacillus megaterium expressing CYP106A2 as whole-cell biocatalyst

Figure 3

Determination of the kinetic parameters for the cyproterone acetate conversion catalyzed by CYP106A2. The reaction kinetics were performed in 50 mM potassium phosphate buffer containing 20% glycerol (pH 7.4) at 30°C for 2 min using 0.5 μM CYP106A2, 10 μM Adx4–108 and 1 μM AdR. Cyproterone acetate was used in a concentration range of 0 to 400 μM. The data shown are the result of four independent measurements (R2>0.98).

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