Figure 3From: Process development for the production of 15β-hydroxycyproterone acetate using Bacillus megaterium expressing CYP106A2 as whole-cell biocatalyst Determination of the kinetic parameters for the cyproterone acetate conversion catalyzed by CYP106A2. The reaction kinetics were performed in 50 mM potassium phosphate buffer containing 20% glycerol (pH 7.4) at 30°C for 2 min using 0.5 μM CYP106A2, 10 μM Adx4–108 and 1 μM AdR. Cyproterone acetate was used in a concentration range of 0 to 400 μM. The data shown are the result of four independent measurements (R2>0.98).Back to article page