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Figure 1 | Microbial Cell Factories

Figure 1

From: Process development for the production of 15β-hydroxycyproterone acetate using Bacillus megaterium expressing CYP106A2 as whole-cell biocatalyst

Figure 1

HPLC chromatogram of the in vitro conversion of cyproterone acetate by CYP106A2. The reaction was performed in 50 mM potassium phosphate buffer containing 20% glycerol (pH 7.4) at 30°C, using 0.5 μM CYP106A2, 10 μM Adx4–108 and 1 μM AdR. The reaction was stopped and extracted twice by ethyl acetate directly after the addition of the substrate (grey dotted line, 0 h) and after 1 h (black line, 1 h). Cyproterone acetate (400 μM) was converted to one main product (P1).

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Microbial Cell Factories

ISSN: 1475-2859

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