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Table 1 Localization of amino acid exchanges of CYP11B1 mutants with increased activity

From: A recombinant CYP11B1 dependent Escherichia coli biocatalyst for selective cortisol production and optimization towards a preparative scale

Amino acid exchange

Localization

H171L

E’-helix, protein surface

Q166R

E’-helix, protein surface

L271M

H-helix, protein surface

S168R

E’-helix, protein surface

M286I

I-helix

Q315E

J-helix, protein surface

  1. Localization of the respective residues discovered during the screening of a random CYP11B1 library for mutants with increased 11β-hydroxylation activity towards 11-deoxycortisol was deduced from the latest homology model of CYP11B1 [25].