Figure 9

Time-dependent whole-cell cortisol formation by the parental CYP11B1 enzyme (Pa1) and the selected mutant L271M in shaking flasks. Pa1 (black circles) was expressed from the Twin_11B1 plasmid carrying 1 Adx copy, while L271M was expressed from plasmids with either 1 Adx (grey cirlces) or 3 Adx (grey triangles) copies. Reactions were performed after a 21-h expression period in TB medium with resting cells in presence of 6% DMSO and 3 mM 11-deoxycortisol. Extracted steroids were analyzed by RP-HPLC. Values represent the mean of three conversion experiments conducted in parallel with respective standard deviation.