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Figure 7 | Microbial Cell Factories

Figure 7

From: A recombinant CYP11B1 dependent Escherichia coli biocatalyst for selective cortisol production and optimization towards a preparative scale

Figure 7

Relative fluorescence developed by recombinant E. coli cultures after incubation with different 11-deoxycortisol concentrations. Cells were transformed with Twin_11B1 and pGro12 and incubated with 11-deoxycortisol for 48 h in TB medium containing 1 mM δ-Ala (white bars) that has been additionally supplemented with 1 mM IPTG and 4 mg/mL arabinose for induction (grey bars). Steroid specific fluorescence was generated by the described assay and measured in relative units (R.F.U.) at λex 485 nm and λem 535 nm. Values represent the mean of triplicates with standard deviations.

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