Figure 6
Comparison of the cortisol formation by recombinant E. coli transformed with different versions of the P450 system encoding plasmid Twin_11B1 with 1 (circles), 2 (squares) or 3 (triangles) copies of the Adx cDNA. Reactions were performed after a 21-h expression period in TB medium with resting cells in the presence of 6% DMSO and 3Â mM 11-deoxycortisol. Extracted steroids were analyzed by RP-HPLC. Values represent the mean of three conversion experiments conducted in parallel with respective standard deviation.