Figure 5
Western blot analysis of Adx expression by recombinant E. coli cells. Lane 1 shows purified bovine Adx4–108 (~11,8 kDa). lane 2 shows a pre-stained protein marker with molecular weights indicated on the left of the picture. Lanes 3 to 6 represent the lysate of E. coli cells expressing the CYP11B1 redox system from variants of the Twin_11B1 plasmid carrying 1 to 4 copies of bovine Adx1–108 (~12,0 kDa). Adx detection was carried out with polyclonal antibodies and a peroxidase-based color reaction. The relative expression level of Adx was determined by measuring the band intensity with the Image Lab software.