Figure 4

Comparison of the whole-cell cortisol formation by CYP11B1 enzyme variants carrying either glycine (G23) or arginine (G23R) in position 23 in recombinant E. coli . Reactions were performed after a 21-h expression period in TB medium with resting cells in the presence of 6% DMSO and 3 mM 11-deoxycortisol. Extracted steroids were analyzed by RP-HPLC. Values represent the mean of three conversion experiments conducted in parallel with respective standard deviation.