Figure 9From: A single freeze-thawing cycle for highly efficient solubilization of inclusion body proteins and its refolding into bioactive form SDS–PAGE analysis of five solubilized proteins from inclusion bodies. Five inclusion body proteins were suspended in PBS buffer (pH 8) containing 2 M urea and followed by freezing at −20°C and thawing at room temperature. Lane M is marker (170, 130, 100, 70, 55, 40, 35, 25, 15 kDa), T stands for cleaned total inclusion bodies, S stands for solubilized protein supernatants from inclusion bodies.Back to article page