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Figure 7 | Microbial Cell Factories

Figure 7

From: A single freeze-thawing cycle for highly efficient solubilization of inclusion body proteins and its refolding into bioactive form

Figure 7

Activity assay of refolded EGFP and MMP-12_CAT from inclusion bodies. (A) Fluorescence spectra of refolded EGFP by freeze-thawing method (Refold EGFP from 2 M urea) and traditional urea-denatured method (Refold EGFP from 8 M urea) compared to native EGFP (Solubly expressed EGFP). (B) Fluorescence increase for the degradation of the Mca-PLGLEEA-Dpa-NH2 (8 uM) by refolded MMP-12_CAT (5 nM) at 25°C from freeze-thawing method and traditional urea-denatured method.

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Microbial Cell Factories

ISSN: 1475-2859

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