Figure 5

Effect of pH on the solubility of EGFP and MMP-12_CAT inclusion bodies by freeze-thawing method. A constant amount of EGFP and MMP-12_CAT inclusion bodies was solubilized at different pH in 20 mM potassium phosphate buffer containing 2 M urea by freeze-thawing method. (A) SDS-PAGE analysis of effect of pH on the solubility of EGFP inclusion bodies. (B) SDS-PAGE analysis of effect of pH on the solubility of MMP-12_CAT inclusion bodies. (C) Protein bands were quantitated by densitometric analysis using ImageJ Software.