Figure 3

SDS-PAGE analysis of EGFP and MMP-12_CAT solubilization from inclusion bodies in PBS buffer containing different molar concentration of urea by two different methods. The same amount of EGFP and MMP-12_CAT inclusion bodies was suspended in PBS buffer at pH 8 containing different molar concentration of urea (0-8 M) and solubilized by freeze-thawing method and traditional urea-denatured method. SDS-PAGE analysis of the solubilization of EGFP from inclusion bodies in different molar concentration of urea by traditional urea-denatured method (A) and freeze-thawing method (B); SDS-PAGE analysis of the solubilization of MMP-12_CAT from inclusion bodies in different molar concentration of urea by traditional urea-denatured method (C) and freeze-thawing method (D). T stands for cleaned total inclusion bodies.