From: Genome reduction boosts heterologous gene expression in Pseudomonas putida
P . putida | Plasmid b | Growth parameters | Protein synthesis | ||
---|---|---|---|---|---|
μ max c (h −1 ) | CDW d (g l −1 ) | π max c (h −1 ) | Y GFP/X c (A.F.U. g CDW −1 ) | ||
KT2440 | None | 0.38 ± 0.01 | 2.6 ± 0.9 | – | – |
pSEVA234 | 0.35 ± 0.02 | 2.1 ± 0.3 | – | – | |
pS234G | 0.28 ± 0.03 | 1.7 ± 0.5 | 0.32 ± 0.06 | 2,125 ± 182 | |
EM329 | None | 0.47 ± 0.02 | 2.9 ± 0.1 | – | – |
pSEVA234 | 0.45 ± 0.01 | 2.9 ± 0.4 | – | – | |
pS234G | 0.42 ± 0.04 | 2.7 ± 0.3 | 0.41 ± 0.02 | 2,613 ± 107 | |
EM383 | None | 0.53 ± 0.01 | 3.4 ± 0.2 | – | – |
pSEVA234 | 0.48 ± 0.02 | 3.1 ± 0.5 | – | – | |
pS234G | 0.46 ± 0.03 | 2.9 ± 0.4 | 0.45 ± 0.01 | 3,047 ± 115 |