Skip to main content

Table 2 Growth and protein synthesis parameters in shaken-flask cultures of different P . putida strains a

From: Genome reduction boosts heterologous gene expression in Pseudomonas putida

P . putida

Plasmid b

Growth parameters

Protein synthesis

μ max c (h −1 )

CDW d (g l −1 )

π max c (h −1 )

Y GFP/X c (A.F.U. g CDW −1 )

KT2440

None

0.38 ± 0.01

2.6 ± 0.9

pSEVA234

0.35 ± 0.02

2.1 ± 0.3

pS234G

0.28 ± 0.03

1.7 ± 0.5

0.32 ± 0.06

2,125 ± 182

EM329

None

0.47 ± 0.02

2.9 ± 0.1

pSEVA234

0.45 ± 0.01

2.9 ± 0.4

pS234G

0.42 ± 0.04

2.7 ± 0.3

0.41 ± 0.02

2,613 ± 107

EM383

None

0.53 ± 0.01

3.4 ± 0.2

pSEVA234

0.48 ± 0.02

3.1 ± 0.5

pS234G

0.46 ± 0.03

2.9 ± 0.4

0.45 ± 0.01

3,047 ± 115

  1. aCells were grown batchwise in M12 minimal medium containing 10 g l−1 glucose as the sole carbon source and 1 mM IPTG was added in the cultures of the recombinant strains as indicated in Methods. Results represent the mean value of the corresponding parameter ± standard deviation of triplicate measurements from at least two independent biological replicates.
  2. bPlasmid pS234G, a derivative of vector pSEVA234, carries gfp under control of an inducible LacIQ/P trc element.
  3. cKinetic parameters were determined during exponential growth. μmax, maximum specific growth rate; πmax, maximum specific rate of GFP formation; Y GFP/X, yield of GFP on biomass; A.F.U., arbitrary fluorescence units; −, not applicable.
  4. dFinal biomass concentration at 24 h. CDW, cell dry weight.