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Table 2 Growth and protein synthesis parameters in shaken-flask cultures of different P . putida strains a

From: Genome reduction boosts heterologous gene expression in Pseudomonas putida

P . putida Plasmid b Growth parameters Protein synthesis
μ max c (h −1 ) CDW d (g l −1 ) π max c (h −1 ) Y GFP/X c (A.F.U. g CDW −1 )
KT2440 None 0.38 ± 0.01 2.6 ± 0.9
pSEVA234 0.35 ± 0.02 2.1 ± 0.3
pS234G 0.28 ± 0.03 1.7 ± 0.5 0.32 ± 0.06 2,125 ± 182
EM329 None 0.47 ± 0.02 2.9 ± 0.1
pSEVA234 0.45 ± 0.01 2.9 ± 0.4
pS234G 0.42 ± 0.04 2.7 ± 0.3 0.41 ± 0.02 2,613 ± 107
EM383 None 0.53 ± 0.01 3.4 ± 0.2
pSEVA234 0.48 ± 0.02 3.1 ± 0.5
pS234G 0.46 ± 0.03 2.9 ± 0.4 0.45 ± 0.01 3,047 ± 115
  1. aCells were grown batchwise in M12 minimal medium containing 10 g l−1 glucose as the sole carbon source and 1 mM IPTG was added in the cultures of the recombinant strains as indicated in Methods. Results represent the mean value of the corresponding parameter ± standard deviation of triplicate measurements from at least two independent biological replicates.
  2. bPlasmid pS234G, a derivative of vector pSEVA234, carries gfp under control of an inducible LacIQ/P trc element.
  3. cKinetic parameters were determined during exponential growth. μmax, maximum specific growth rate; πmax, maximum specific rate of GFP formation; Y GFP/X, yield of GFP on biomass; A.F.U., arbitrary fluorescence units; −, not applicable.
  4. dFinal biomass concentration at 24 h. CDW, cell dry weight.