Skip to main content

Advertisement

Table 1 Bacterial strains and plasmids used in this study

From: Genome reduction boosts heterologous gene expression in Pseudomonas putida

Bacterial strain or plasmid Relevant characteristics a Source or reference
E. coli
DH5α Cloning host; F λ endA1 glnX44(AS) thiE1 recA1 relA1 spoT1 gyrA96(NalR) rfbC1 deoR nupG Φ80(lacZΔM15) Δ(argF-lac)U169 hsdR17(r K m K +) [27]
Pseudomonas putida
KT2440 Wild-type strain, spontaneous restriction-deficient derivative of strain mt-2 cured of the TOL plasmid pWW0 [28]
EM329 Flagella-less derivative of KT2440; ΔPP4329-PP4397 (flagellar operon) [23]
EM383 Streamlined derivative of KT2440; ΔPP4329-PP4397 (flagellar operon) ΔPP3849-PP3920 (prophage 1) ΔPP3026-PP3066 (prophage 2) ΔPP2266-PP2297 (prophage 3) ΔPP1532-PP1586 (prophage 4) ΔTn7 ΔendA-1 ΔendA-2 ΔhsdRMS Δflagellum ΔTn4652 [25]
Plasmid
pSEVA234b Expression vector; oriV(pBBR1) lacI Q P trc aphA, KmR [22]
pSEVA637b Cloning vector carrying the green fluorescent protein gene; oriV(pBBR1) aacC1, GmR [22]
pS234G Expression vector carrying the green fluorescent protein gene under control of the inducible P trc promoter; oriV(pBBR1) lacI Q P trc gfp aphA, KmR This study
  1. aAntibiotic markers: Gm, gentamicin; Km, kanamycin.
  2. bPlasmids belonging to the SEVA (Standard European Vector Architecture) collection.