Table 1 Bacterial strains and plasmids used in this study
From: Genome reduction boosts heterologous gene expression in Pseudomonas putida
Bacterial strain or plasmid | Relevant characteristics a | Source or reference |
|---|---|---|
E. coli | ||
DH5α | Cloning host; F− λ− endA1 glnX44(AS) thiE1 recA1 relA1 spoT1 gyrA96(NalR) rfbC1 deoR nupG Φ80(lacZΔM15) Δ(argF-lac)U169 hsdR17(r K − m K +) | [27] |
Pseudomonas putida | ||
KT2440 | Wild-type strain, spontaneous restriction-deficient derivative of strain mt-2 cured of the TOL plasmid pWW0 | [28] |
EM329 | Flagella-less derivative of KT2440; ΔPP4329-PP4397 (flagellar operon) | [23] |
EM383 | Streamlined derivative of KT2440; ΔPP4329-PP4397 (flagellar operon) ΔPP3849-PP3920 (prophage 1) ΔPP3026-PP3066 (prophage 2) ΔPP2266-PP2297 (prophage 3) ΔPP1532-PP1586 (prophage 4) ΔTn7 ΔendA-1 ΔendA-2 ΔhsdRMS Δflagellum ΔTn4652 | [25] |
Plasmid | ||
pSEVA234b | Expression vector; oriV(pBBR1) lacI Q P trc aphA, KmR | [22] |
pSEVA637b | Cloning vector carrying the green fluorescent protein gene; oriV(pBBR1) aacC1, GmR | [22] |
pS234G | Expression vector carrying the green fluorescent protein gene under control of the inducible P trc promoter; oriV(pBBR1) lacI Q P trc →gfp aphA, KmR | This study |