Table 4 Primers used in this study
From: Construction of efficient xylose utilizing Pichia pastoris for industrial enzyme production
Primer name | Primer sequences (5′-3′) | Size (bp) |
|---|---|---|
5′ GAP | gtccctatttcaatcaattgaa | 22 |
3′ AOX | gcaaatggcattctgacatcc | 21 |
XI-F | atcaagaattcatgactaagga | 22 |
XI-R | aaagctggcggccgcttactgat | 23 |
XK-F | atcggaattcatggttaccaaagaaatccaaa | 32 |
XK-R | attggcggccgcaaacgcctgacttgcttcac | 32 |
rACT1-F | agtgttcccatcggtcgtag | 20 |
rACT1-R | ggtgtggtgccagatctttt | 20 |
rXI-F | catacgttatgcgctgatgg | 20 |
rXI-R | ccctcgctcactagatcgac | 20 |
rXK-F | tcttcatggcaaggaggaac | 20 |
rXK-R | atcgaagacggcatgatagg | 20 |
rXR-F | taccatcaccctgacaacgt | 20 |
rXR-R | atccaccaacctctctagcg | 20 |
rXDH-F | cccgtctcgttacagcaatg | 20 |
rXDH-R | gcatggacagcaacactcaa | 20 |