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Table 3 Changes of copy numbers of xylose pathway genes after evolutionary engineering

From: Construction of efficient xylose utilizing Pichia pastoris for industrial enzyme production

Strains

XI

XK

XDH

GS-XISB50/GS-XI

6.17 ± 0.76

N.D

N.D

GS-XKSB50/GS-XK

—

0.84 ± 0.27

N.D

GS-XI-XKSB50/GS-XI-XK

1.27 ± 0.07

N.D

1.03 ± 0.19

  1. Copy number was determined by real time qPCR. ACT1 gene was utilized as a reference control. Each sample was run in triplicates.
  2. N.D: not determined, because no significant transcriptional changes were observed.
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Microbial Cell Factories

ISSN: 1475-2859

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