Construction of efficient xylose utilizing Pichia pastoris for industrial enzyme production

Table 2 The calculated fermentation performance metrics for all engineered Pichia pastoris strains

Strains	μ (h ⁻¹ )	q _s (g/g DCW/h)	Y _X/S (g/g DCW)
GS115	0.0075 ± 0.0008	0.056 ± 0.006	0.135 ± 0.004
GS-XI	0.0107 ± 0.0014	0.055 ± 0.007	0.194 ± 0.007
GS-XK	0.0072 ± 0.0007	0.059 ± 0.008	0.123 ± 0.012
GS-XI-XK	0.0103 ± 0.0014	0.057 ± 0.008	0.181 ± 0.010
GS115^SB50	0.0112 ± 0.0005	0.061 ± 0.006	0.185 ± 0.015
GS-XI^SB50	0.0193 ± 0.0013	0.051 ± 0.004	0.378 ± 0.013
GS-XK^SB50	0.0138 ± 0.0007	0.051 ± 0.006	0.271 ± 0.027
GS-XI-XK^SB50	0.0110 ± 0.0013	0.092 ± 0.011	0.120 ± 0.003

μ, specific growth rate; q_s, specific xylose consumption rate; Y_X/S, cell yield on xylose. All metrics were average values calculated based on fermentation data at 72 h of fermentation. The contribution of oligopeptides present in the complex medium to performance metrics was excluded based on data from Figure 1.

ISSN: 1475-2859