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Table 2 List of primers used in PCR experiments a

From: Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum by expressing glutamate decarboxylase active in expanded pH range

Primer name

Primer sequence (5′ to 3′)

Gad-del-C-F

tgacgaggatccatgggcagcagcc

Gad-del-C-R

ctgctatctagattagtgatcgctgagatatttca

I16-F

tcgatcggtaccagacaccgc

I16-R

gatcgaggatcccaataatatcctgtagc

L26-F

tcgatcggtaccgtgagtttagagca

L26-R

gatcgaggatccgatagtaatcctaacg

Gad-wt-R

tgctagcggccgcttaggtatgtttaaagctgttctgtt

  1. aRestriction enzyme sites are shown in bold.