Table 1 Bacterial strains and plasmids used in this study

E. coli XL1-Blue

recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proABlacIq ZΔM15 Tn10 (Tet ^r )]

Stratagene^a

E. coli DH5α

fhuA2 lac(del)U169 phoA glnV44 Φ80' lacZ(del)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

Lab stock

C. glutamicum

Wild type

ATCC13032

Plasmids

Relevant characteristics

Reference or source

pET28b-GAD ΔCE89Q

gadB mutant (Glu89Gln/Δ452-466 gene)

[20]

pCES208

E. coli-C. Glutamicum shuttle vector, Km^r

[40]

pCES-H36-GFP

pCES208 derivative, P_H36, eGFP

[21]

pCES-I16-GFP

pCES208 derivative, P_I16, eGFP

[21]

pCES-L26-GFP

pCES208 derivative, P_L26, eGFP

[21]

pHGwt

pCES208 derivative, gadB (wt)

This study

pHGmut

pCES208 derivative, Glu89Gln/Δ452-466 gene

This study

pLGmut

pCES208 derivative, P_L26, Glu89Gln/Δ452-466 gene

This study

pIGmut

pCES208 derivative, P_I16, Glu89Gln/Δ452-466 gene

This study