Figure 3

Time profiles of cell density and GABA concentrations during flask cultivations with different biotin concentrations. Square, circle, triangle and diamond symbols indicate 1 μg/L, 50 μg/L, 100 μg/L, and 500 μg/L, respectively. (A) Time profiles of cell growth at four different biotin concentration conditions. (B) Open and closed symbols indicate the L-glutamate concentrations and GABA concentrations, respectively. Each point represents the average of three independent experiments. Each point represents the average of three independent experiments.