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Table 3 Bacterial strains and plasmids used in this study

From: A strategy for enhanced circular DNA construction efficiency based on DNA cyclization after microbial transformation

Strains and plasmids

Description

Reference(s) and/or derivations

E. coli S17-1λ pir

TpR SmR recA, thi, pro, hsdR-M + RP4: 2-Tc:Mu: Km Tn7 λp

19

E. coli BL21(DE3)

F−, ompT, hsdS(rb −mb −), gal, dcm(DE3)

Transgene Corporation

E. coli TSA

Host strain fro linear plasmid

Lucigen corporation

A. hydrophila 4AK4

AmpR; Wild type isolated from sewage samples, PHBHHx producing strain

22

P. denitrificans

Gram-negative facultative anaerobic bacterium that performs denitrification, producing VB12

Huabei Pharmaceutical Enterprise

pUK

HK022 attP site, KmR

23

pUKG

pUK derived, attL,attR sequence produced by recombination, KmR

This study

pCP20

FLP recombinase helper plasmid, ts-rep, AmpR,CmR

21

pAH83CI

pAH83 derived, expression control gene cI was broken by HindIII digesition

23

pAH69

pAL2 derived,int under the control of P1 promoter, expression control gene cI AmpR

E.coli Genetic Stock Center

pJAZZOC

Cloning vector

Lucigen corporation

pJAZZG

pJAZZOC derived, a fragment containing DraIII recognition site inserted.

This study

pVQL

P15A replicon, CmR

Qing Lan corporation

p15ABCDAI

pVQL derived, haboring genes hemBCD, cobAI, CmR

This study