Strains and plasmids | Description | Reference(s) and/or derivations |
---|---|---|
E. coli S17-1λ pir | TpR SmR recA, thi, pro, hsdR-M + RP4: 2-Tc:Mu: Km Tn7 λp | 19 |
E. coli BL21(DE3) | F−, ompT, hsdS(rb −mb −), gal, dcm(DE3) | Transgene Corporation |
E. coli TSA | Host strain fro linear plasmid | Lucigen corporation |
A. hydrophila 4AK4 | AmpR; Wild type isolated from sewage samples, PHBHHx producing strain | 22 |
P. denitrificans | Gram-negative facultative anaerobic bacterium that performs denitrification, producing VB12 | Huabei Pharmaceutical Enterprise |
pUK | HK022 attP site, KmR | 23 |
pUKG | pUK derived, attL,attR sequence produced by recombination, KmR | This study |
pCP20 | FLP recombinase helper plasmid, ts-rep, AmpR,CmR | 21 |
pAH83CI | pAH83 derived, expression control gene cI was broken by HindIII digesition | 23 |
pAH69 | pAL2 derived,int under the control of P1 promoter, expression control gene cI AmpR | E.coli Genetic Stock Center |
pJAZZOC | Cloning vector | Lucigen corporation |
pJAZZG | pJAZZOC derived, a fragment containing DraIII recognition site inserted. | This study |
pVQL | P15A replicon, CmR | Qing Lan corporation |
p15ABCDAI | pVQL derived, haboring genes hemBCD, cobAI, CmR | This study |