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Table 2 Results of the comparisons between CAT strategy and non-CAT control

From: A strategy for enhanced circular DNA construction efficiency based on DNA cyclization after microbial transformation

Ligation

0.5 pM

1 pM

2 pM

Circular Recombinant DNA

38 (35/38)

184 (44/48)

304 (40/48)

CAT - Recombinant DNA

88 (44/48)

364 (35/48)

804 (42/48)

CAT /non- CAT × 4/3

3.07

2.10

3.70

Circular Gibson Assembly

84 (37/48)

168 (32/48)

284 (32/48)

CAT - Gibson Assembly

140 (40/48)

308 (33/48)

624 (38/48)

CAT /non- CAT × 4/3

2.40

2.52

3.48

Circular SLIC

66 (20/48)

142 (25/48)

276 (28/48)

CAT -SLIC

124 (38/48)

212 (41/48)

486 (40/48)

CAT /non- CAT × 4/3

4.76

3.26

3.48

Circular Golden Gate

N/A

4 (3/4)

25 (10/25)

CAT - Golden Gate

N/A

132 (40/48)

248 (29/48)

  1. The comparisons between CAT and non-CAT control (explained in Figure 4) were presented by the colony number and correct colony number. CAT/non-CAT is the correct colony number in CAT over the correct colony number in non-CAT.