Ligation | 4 DNA fragments | 6 DNA fragments |
---|---|---|
Conventional in vitro circular ligation system (S17-1λ pir ) | 204(16/96) | 60(2/60) |
In vitro diluted self-ligation cyclization (S17-1λ pir ) | 162(42/96) | 22 (12/22) |
In vitro self-recombination cyclization (S17-1λ pir ) | ---- | 40(3/40) |
CAT strategy (S17-1λ pir∆attB HK022 pAH83CI) | 200(80/96) | 42(36/42) |