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Table 1 Number of transformants formed using conventional in vitro circular ligation system, in vitro diluted self-ligation cyclization, in vitro self-recombination cyclization, and CAT , respectively

From: A strategy for enhanced circular DNA construction efficiency based on DNA cyclization after microbial transformation

Ligation

4 DNA fragments

6 DNA fragments

Conventional in vitro circular ligation system (S17-1λ pir )

204(16/96)

60(2/60)

In vitro diluted self-ligation cyclization (S17-1λ pir )

162(42/96)

22 (12/22)

In vitro self-recombination cyclization (S17-1λ pir )

----

40(3/40)

CAT strategy (S17-1λ pir∆attB HK022 pAH83CI)

200(80/96)

42(36/42)