Figure 3

Schematic description of various attempts to avoid the contradiction on bimolecular ligation/assembly and monomolecular cyclization. A) Conventional circular ligation, B) Ligation into linear plasmid. No cyclization is required to make the plasmid stable and thus the contradiction was avoided. C) Linear ligation and diluted self-ligating cyclization (First branch from the left), linear ligation and in vitro LR clonase cyclization (Second branch from the left) linear ligation coupled with in vivo cyclization (Third branch from the left).