Figure 5

Effect of repeat number of the single amino acid between K and E on secretory activities. a) The poly-I, F, and M sequence was changed to 10–17, 8–17, and 10–17, respectively and the activities were compared with WT signal sequence. Data were shown as in Figure 2. b) RT-PCR of the sequenced clones for WT (RAK6205), M¹⁶ (RAK8772), F¹³ (RAK9383) and L¹³ (RAK10336). Amplified cDNA bands of yGLuc and the control ACT1 at 30, 35, 40 cycles are shown. c) Western blotting analysis of the supernatants from WT (RAK6205) and M¹⁶ (RAK8772) using anti-GLuc antibody.