Figure 2

Effect of amino acid substitution in the GLuc signal sequence on secretion activity. a) Mutagenesis of the 16th Glu (E). E16Δ is the deletion mutant and the others are substitutions to other amino acids. b) Mutagenesis of the 4th Lys (K), with deletion of the 2nd Gly and 3rd Val (GVΔ). Activity is shown as fold change calculated as the ratio of mutagenized GLuc activity per wild type activity. The error bars indicate SEs of at least 5 (a) or 6 (b) transformants except for null-activity ones.