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Figure 3 | Microbial Cell Factories

Figure 3

From: Systematic comparison of single-chain Fv antibody-fusion toxin constructs containing Pseudomonas Exotoxin A or saporin produced in different microbial expression systems

Figure 3

Characterization of recombinant ITs expressed in E. coli purified by IMAC. (A) Coomassie staining and (B) Western blot with anti-His antibody of purified 4KB-PE40 in lane 1, 4KB(218)-PE40 in lane 2 and 4KB(218)-SAP in lane 3. (C) Comparison of the binding characteristics of 4KB-PE40 (blue diamonds), 4KB(218)-PE40 (green circles) and 4KB(218)-SAP (red triangles) analyzed by flow-cytometry using Daudi cells incubated at 4°C with increasing concentrations of each IT.

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Microbial Cell Factories

ISSN: 1475-2859

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