Skip to main content

Table 2 Primers and annealing temperatures used in real–time PCR

From: Improved production of sublancin via introduction of three characteristic promoters into operon clusters responsible for this novel distinct glycopeptide biosynthesis

Gene

Primer

Sequence

Annealing temperature (°C)

sunI

sunI–up

AAGAGTCAGACAAGTATGGAGTT

48

 

sunI–down

TTAAATGGAGCTCAACAATTTA

 

sunA

sunA–up

GAACTGGAAAATCAAAAAGGT

49

 

sunA–down

CAAAACTGCCGGTAATTCT

 

sunT

sunT–up

GGGGATAAGGAAGGCTATAG

50

 

sunT–down

TAATGTCCATATTCCTCCCC

 

bdbA

bdbA–up

GCAGCAGCCATTAGTATTTTC

51

 

bdbA–down

CAAGGAGGACAACTTGTCTCA

 

sunS

sunS–up

GGCTATGCCGATTCTTTATT

50

 

sunS–down

CCGCATGTTATTGTAGGAGTA

 

bdbB

bdbB–up

CCATGTGTTCTATGTTGGTATC

49

 

bdbB–down

CCAATTTCACATACGACACTT

Â