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Table 1 The bacterial strains and vectors used in the bioengineering of bacteria to produce higher alcohols

From: Engineering Corynebacterium crenatum to produce higher alcohols for biofuel using hydrolysates of duckweed (Landoltia punctata) as feedstock

Strain or plasmid

Relevant genotype

Source

Strains

DH5α

F, φ 80dlacZ ΔM15, Δ(lacZYA-argF )U169, deoR, recA1, endA1, hsdR17(rK , mK +), phoA, supE44, λ ,thi −1, gyrA96, relA1

Takara: 9057

C. crenatum

ompT, hsdSB (rB mB ),gal, dcm

CICC 20153

Plasmids

  

pSTV29

pACYC184 ori; Cmr; PLlacO−1: MCS

Takara: 3332

p29-pBL

pBL1ori; Cmr; PLlacO−1: MCS

This study

PEC-XK99E

pGA1 Kmr pTrc99A MCS P-trc,lacIq

From CAS

pXMJ19

pBL1ori; Kmr; MCS P-trc, lacIq

From CAS

PEC-KA

pGA1 Kmr pTrc99A MCS P-trc, lacIq: Kivd(LL)-ADH2(SC)

This study

p29- AL

pBL1ori; Cmr; PLlacO-1:MCS:LEU2(SC)

This study

p29- ALI

pBL1ori; Cmr; PLlacO-1: LEU2(SC)-ILV2(SC)

This study

p29- ALII

pBL1ori; Cmr; PLlacO-1: LEU2(SC)-ILV2-ILV5(SC)

This study

p29- BI

pBL1ori; Cmr; PLlacO-1: ILV2(SC)

This study

p29- BII

pBL1ori; Cmr; PLlacO−1: ILV2(SC)-ILV5(SC)

This study

p29- BIII

pBL1ori; Cmr; PLlacO−1: ILV2(SC)-ILV5(SC)-ILV3(SC)

This study

p29- CB

pBL1ori; Cmr; PLlacO−1: BAT2(SC)

This study

p29-DL

pBL1ori; Cmr; PLlacO−1: LEU4(SC)

This study

p29-DLL

pBL1ori; Cmr; PLlacO−1:LEU4(SC)-LEU1(SC)

This study

p29-FL

pBL1ori; Cmr; PLlacO−1: LEU1(SC)

This study

  1. SC: S. cerevisiae AH109; LL: Lactococcus lactis subsp. cremoris CICC 1605.