|
Strains
|
|
DH5α
|
F−, φ 80dlacZ ΔM15, Δ(lacZYA-argF )U169, deoR, recA1, endA1, hsdR17(rK
−, mK
+), phoA, supE44, λ
−,thi
−1, gyrA96, relA1
|
Takara: 9057
|
|
C. crenatum
|
ompT, hsdSB (rB
−
mB
−
),gal, dcm
|
CICC 20153
|
|
Plasmids
| | |
|
pSTV29
|
pACYC184 ori; Cmr; PLlacO−1: MCS
|
Takara: 3332
|
|
p29-pBL
|
pBL1ori; Cmr; PLlacO−1: MCS
|
This study
|
|
PEC-XK99E
|
pGA1 Kmr pTrc99A MCS P-trc,lacIq
|
From CAS
|
|
pXMJ19
|
pBL1ori; Kmr; MCS P-trc, lacIq
|
From CAS
|
|
PEC-KA
|
pGA1 Kmr pTrc99A MCS P-trc, lacIq: Kivd(LL)-ADH2(SC)
|
This study
|
|
p29- AL
|
pBL1ori; Cmr; PLlacO-1:MCS:LEU2(SC)
|
This study
|
|
p29- ALI
|
pBL1ori; Cmr; PLlacO-1: LEU2(SC)-ILV2(SC)
|
This study
|
|
p29- ALII
|
pBL1ori; Cmr; PLlacO-1: LEU2(SC)-ILV2-ILV5(SC)
|
This study
|
|
p29- BI
|
pBL1ori; Cmr; PLlacO-1: ILV2(SC)
|
This study
|
|
p29- BII
|
pBL1ori; Cmr; PLlacO−1: ILV2(SC)-ILV5(SC)
|
This study
|
|
p29- BIII
|
pBL1ori; Cmr; PLlacO−1: ILV2(SC)-ILV5(SC)-ILV3(SC)
|
This study
|
|
p29- CB
|
pBL1ori; Cmr; PLlacO−1: BAT2(SC)
|
This study
|
|
p29-DL
|
pBL1ori; Cmr; PLlacO−1: LEU4(SC)
|
This study
|
|
p29-DLL
|
pBL1ori; Cmr; PLlacO−1:LEU4(SC)-LEU1(SC)
|
This study
|
|
p29-FL
|
pBL1ori; Cmr; PLlacO−1: LEU1(SC)
|
This study
|
