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Table 1 The bacterial strains and vectors used in the bioengineering of bacteria to produce higher alcohols

From: Engineering Corynebacterium crenatum to produce higher alcohols for biofuel using hydrolysates of duckweed (Landoltia punctata) as feedstock

Strain or plasmid Relevant genotype Source
Strains
DH5α F, φ 80dlacZ ΔM15, Δ(lacZYA-argF )U169, deoR, recA1, endA1, hsdR17(rK , mK +), phoA, supE44, λ ,thi −1, gyrA96, relA1 Takara: 9057
C. crenatum ompT, hsdSB (rB mB ),gal, dcm CICC 20153
Plasmids   
pSTV29 pACYC184 ori; Cmr; PLlacO−1: MCS Takara: 3332
p29-pBL pBL1ori; Cmr; PLlacO−1: MCS This study
PEC-XK99E pGA1 Kmr pTrc99A MCS P-trc,lacIq From CAS
pXMJ19 pBL1ori; Kmr; MCS P-trc, lacIq From CAS
PEC-KA pGA1 Kmr pTrc99A MCS P-trc, lacIq: Kivd(LL)-ADH2(SC) This study
p29- AL pBL1ori; Cmr; PLlacO-1:MCS:LEU2(SC) This study
p29- ALI pBL1ori; Cmr; PLlacO-1: LEU2(SC)-ILV2(SC) This study
p29- ALII pBL1ori; Cmr; PLlacO-1: LEU2(SC)-ILV2-ILV5(SC) This study
p29- BI pBL1ori; Cmr; PLlacO-1: ILV2(SC) This study
p29- BII pBL1ori; Cmr; PLlacO−1: ILV2(SC)-ILV5(SC) This study
p29- BIII pBL1ori; Cmr; PLlacO−1: ILV2(SC)-ILV5(SC)-ILV3(SC) This study
p29- CB pBL1ori; Cmr; PLlacO−1: BAT2(SC) This study
p29-DL pBL1ori; Cmr; PLlacO−1: LEU4(SC) This study
p29-DLL pBL1ori; Cmr; PLlacO−1:LEU4(SC)-LEU1(SC) This study
p29-FL pBL1ori; Cmr; PLlacO−1: LEU1(SC) This study
  1. SC: S. cerevisiae AH109; LL: Lactococcus lactis subsp. cremoris CICC 1605.