Strain or plasmid | Characteristic (s) a | Reference or source |
---|---|---|
Reference or source | ||
Strains | ||
E. coli TG1 | supE, hsd, Δ5, thi, Δ(lac-proAB), F’(traD36 proAB-lacZΔM15) | [23] |
L. lactis MG1363 | Wild type strain, plasmid free | [24] |
L. lactis NZ9000 | MG1363 (nisRK genes integrated into chromosome), plasmid free | [25] |
L. lactis NZ9000 htrA | NZ9000 carrying htrA disruption (double-crossover recombination) | [22] |
Plasmids | ||
pCR-TOPO | ApR, subloning TOPO vector | Invitrogen |
pORF9-mTGF-β1 | ApR, pORF9 plasmid carrying murine TGF-β1 gene | InvivoGen |
pDK6-mSLPI | ApR, pDK6 plasmid carrying full length murine secretory leukocyte protease inhibitor (SLPI) cDNA under the control of the MCMV promoter | [26] |
pSEC:Nuc | CmR, pGK plasmid (a derivative from the broad host range plasmid pWV01) expressing a secreted form of the staphylococcal nuclease (Nuc) under the control of P nisA pomoter | [27] |
pSEC:mIL-10 | CmR, pGK plasmid expressing a secreted form of murine IL-10 cytokine under the control of P nisA promoter | [17] |
pSEC:elafin | CmR, pGK plasmid expressing a secreted form of human elafin antiprotease under the control of P nisA pomoter | [17] |
pSEC:mTGF-β | CmR, pGK plasmid expressing a secreted form of murine TGF-β cytokine under the control of P nisA pomoter | This study |
pSEC: mSLPI | CmR, pGK plasmid expressing a secreted form of murine SLPI antiprotease under the control of P nisA pomoter | This study |