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Table 1 Bacterial strains and plasmids used in this study

From: Serine protease inhibitors protect better than IL-10 and TGF-β anti-inflammatory cytokines against mouse colitis when delivered by recombinant lactococci

Strain or plasmid

Characteristic (s) a

Reference or source

 

Reference or source

 

Strains

  

E. coli TG1

supE, hsd, Δ5, thi, Δ(lac-proAB), F’(traD36 proAB-lacZΔM15)

[23]

L. lactis MG1363

Wild type strain, plasmid free

[24]

L. lactis NZ9000

MG1363 (nisRK genes integrated into chromosome), plasmid free

[25]

L. lactis NZ9000 htrA

NZ9000 carrying htrA disruption (double-crossover recombination)

[22]

Plasmids

  

pCR-TOPO

ApR, subloning TOPO vector

Invitrogen

pORF9-mTGF-β1

ApR, pORF9 plasmid carrying murine TGF-β1 gene

InvivoGen

pDK6-mSLPI

ApR, pDK6 plasmid carrying full length murine secretory leukocyte protease inhibitor (SLPI) cDNA under the control of the MCMV promoter

[26]

pSEC:Nuc

CmR, pGK plasmid (a derivative from the broad host range plasmid pWV01) expressing a secreted form of the staphylococcal nuclease (Nuc) under the control of P nisA pomoter

[27]

pSEC:mIL-10

CmR, pGK plasmid expressing a secreted form of murine IL-10 cytokine under the control of P nisA promoter

[17]

pSEC:elafin

CmR, pGK plasmid expressing a secreted form of human elafin antiprotease under the control of P nisA pomoter

[17]

pSEC:mTGF-β

CmR, pGK plasmid expressing a secreted form of murine TGF-β cytokine under the control of P nisA pomoter

This study

pSEC: mSLPI

CmR, pGK plasmid expressing a secreted form of murine SLPI antiprotease under the control of P nisA pomoter

This study

  1. aFor strains, genotypic and phenotypic characteristics are given; for plasmids, plasmid and cloned-cassette characteristics are given.