Figure 5

Analysis of differential allelic expression (DAE). A- Allelic expression frequency of the genes located on chromosome 13 (Chr13). The allele with higher expression was arbitrarily designated as allele 1 (blue) and the lower expression allele was designated as allele 2 (red). The hatched box represents a 100-kb homozygous region (including the centromere) in the JAY270/PE2 diploid where no DAE information could be assessed. The DAE plot suggests that the breakpoint of the Chr13 LOH event occurred within the homozygous region. The position of the RCE1 locus on the right arm is shown. B- The genotypes at the RCE1 locus were determined by PCR followed by restriction analysis using MboI. The predicted banding patterns for the alleles were: Homozygous for allele 1 (cut) 543 bp and 256 bp; Homozygous for allele 2 (uncut) 799 bp; Heterozygous pattern: 799 bp, 543 bp and 256 bp. A molecular weight marker ladder of 100 bp incremental size fragments was used in line 1. The 500 bp marker band is indicated. C- Venn diagram showing the number of DAE genes identified exclusively in the TF and FL, and the DAE genes identified in simultaneously in both TF and FL.