In vivo production of a novel glycoconjugate vaccine against Shigella flexneri 2a in recombinant Escherichia coli: identification of stimulating factors for in vivo glycosylation

Table 2 Comparison of specific and volumetric EPA-2a yields in shake flask and high cell density cultivations

Type of cultivation	Induction strategy	N-acetylglucosamine g/L	OD ₆₀₀ at induction	Final OD ₆₀₀	Specific yield (A450 nm/OD ₆₀₀ )*	Volumetric yield (A450 nm/volume)	X-fold increase
Shake flask	Simultaneous	-	0.8	1.9	0.38	0.72	-
Shake flask	Sequential	10	0.8	2.6	1.00	2.6	3.6
Bioreactor fed-batch	Sequential	10	9.1	41.1	0.82	33.7	46.2

ISSN: 1475-2859