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Figure 5 | Microbial Cell Factories

Figure 5

From: In vivo production of a novel glycoconjugate vaccine against Shigella flexneri 2a in recombinant Escherichia coli: identification of stimulating factors for in vivo glycosylation

Figure 5

Mg 2+ stimulates EPA-2a expression. A) Culture medium was supplemented with different amounts of Mg2+, and EPA-2a was quantified in OD600-normalized periplasmic extracts by ELISA after incubation at 30°C and simultaneous induction with 1 mM IPTG and 2 g/L arabinose for 24 h in 96-deep well plates. B) Influence of different amounts of Mg2+ on the final OD600 values i in 96-deep well plates. Error bars represent the standard deviations of three biological replicates.

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Microbial Cell Factories

ISSN: 1475-2859

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